phosphorimager model 425s (Molecular Dynamics Inc)
90
Structured Review
Molecular Dynamics Inc
phosphorimager model 425s
![A, a specific 32P-labeled phosphoprotein, generated during a 5-min incubation of Kdo2-[4′-32P]lipid IVA with enzyme (as described under “Experimental Procedures”) is resolved by SDS-polyacrylamide gel electrophoresis and detected by <t>PhosphorImager</t> analysis of the dried gel. Partially purified enzyme from either the Q-Sepharose step (lane 2, 6 μg of protein) or the heparin-agarose step (lanes 1 and 3; 2 μg of protein) was used. The enzyme for the reaction shown in lane 1 was first heated to 65 °C for 15 min. B, PtdIns reduces the level of the phosphoprotein generated during a 5-min incubation of Kdo2-[4′-32P]lipid IVA with enzyme. Reactions were performed with identical amounts (2 μg) of heparin-agarose enzyme and Kdo2-[4′-32P]lipid IVA in the absence (lane 1) or in the presence (lane 2) of PtdIns (0.5 mg/ml). After 5 min, the reactions were quenched with SDS and subjected to gel electrophoresis.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_8417/pmc02548417/pmc02548417__nihms66802f11.jpg)
Phosphorimager Model 425s, supplied by Molecular Dynamics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phosphorimager+model+425/phosphorimager+screen/pmc02548417-108-29-27
Average 90 stars, based on 1 article reviews
Phosphorimager Model 425s, supplied by Molecular Dynamics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phosphorimager+model+425/phosphorimager+screen/pmc02548417-108-29-27
Average 90 stars, based on 1 article reviews
phosphorimager model 425s - by Bioz Stars,
2026-09
90/100 stars
Images
1) Product Images from "A Phosphotransferase That Generates Phosphatidylinositol 4-Phosphate (PtdIns-4-P) from Phosphatidylinositol and Lipid A in Rhizobium leguminosarum.: A MEMBRANE-BOUND ENZYME LINKING LIPID A AND PtdIns-4-P BIOSYNTHESIS * "
Article Title: A Phosphotransferase That Generates Phosphatidylinositol 4-Phosphate (PtdIns-4-P) from Phosphatidylinositol and Lipid A in Rhizobium leguminosarum.: A MEMBRANE-BOUND ENZYME LINKING LIPID A AND PtdIns-4-P BIOSYNTHESIS
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Figure Legend Snippet: A, a specific 32P-labeled phosphoprotein, generated during a 5-min incubation of Kdo2-[4′-32P]lipid IVA with enzyme (as described under “Experimental Procedures”) is resolved by SDS-polyacrylamide gel electrophoresis and detected by PhosphorImager analysis of the dried gel. Partially purified enzyme from either the Q-Sepharose step (lane 2, 6 μg of protein) or the heparin-agarose step (lanes 1 and 3; 2 μg of protein) was used. The enzyme for the reaction shown in lane 1 was first heated to 65 °C for 15 min. B, PtdIns reduces the level of the phosphoprotein generated during a 5-min incubation of Kdo2-[4′-32P]lipid IVA with enzyme. Reactions were performed with identical amounts (2 μg) of heparin-agarose enzyme and Kdo2-[4′-32P]lipid IVA in the absence (lane 1) or in the presence (lane 2) of PtdIns (0.5 mg/ml). After 5 min, the reactions were quenched with SDS and subjected to gel electrophoresis.
Techniques Used: Labeling, Generated, Incubation, Polyacrylamide Gel Electrophoresis, Purification, Nucleic Acid Electrophoresis
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